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The Influence of Virus Infection the Extracellular pH of the Host Cell Detected on Cell Membrane

By April 13, 2020Infectious Disease

The Influence of Virus Infection the Extracellular pH of the Host Cell Detected on Cell Membrane

Hengjun Liu 1*, HisatakaMaruyama1, TaisukeMasuda1, AyaeHonda2 and FumihitoArai 1
1 DepartmentofMicro-NanoSystemsEngineering,NagoyaUniversity,Nagoya,Japan, 2 DepartmentofFrontierBioscience,
HoseiUniversity,Tokyo,Japan
Influenzavirusinfectioncanresultinchangesinthecellularionlevelsat2–

Influenza virus infection can result in changes in the cellular ion levels at 2–3h post infection. More H+ is produced by glycolysis, and the viral M2 proton channel also plays a role in the capture and release of H+ during both viral entry and egress.  Then the cells might regulate the intracellular pH by increasing the export of H+ from the intracellular compartment. Increased H+ export could lead indirectly to increased extracellular acidity.  To detect changes in extracellular pH of both virus infected and uninfected cells, pH sensors were synthesized using polystyrene beads(#1μm) containing Rhodamine B and Fluorescein isothiocyanate (FITC). The fluorescence intensity of FITC can respond to both pH and temperature.

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