The Influence of Virus Infection the Extracellular pH of the Host Cell Detected on Cell Membrane
Hengjun Liu 1*, HisatakaMaruyama1, TaisukeMasuda1, AyaeHonda2 and FumihitoArai 1
1 DepartmentofMicro-NanoSystemsEngineering,NagoyaUniversity,Nagoya,Japan, 2 DepartmentofFrontierBioscience,
Influenza virus infection can result in changes in the cellular ion levels at 2–3h post infection. More H+ is produced by glycolysis, and the viral M2 proton channel also plays a role in the capture and release of H+ during both viral entry and egress. Then the cells might regulate the intracellular pH by increasing the export of H+ from the intracellular compartment. Increased H+ export could lead indirectly to increased extracellular acidity. To detect changes in extracellular pH of both virus infected and uninfected cells, pH sensors were synthesized using polystyrene beads(#1μm) containing Rhodamine B and Fluorescein isothiocyanate (FITC). The fluorescence intensity of FITC can respond to both pH and temperature.