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Monthly Archives

March 2018

The molecular era of the mitochondrial calcium uniporter

By | Research Study

The molecular era of the mitochondrial calcium uniporter

Kimberli J. Kamer1,2 and Vamsi K. Mootha2–4

Abstract | The mitochondrial calcium uniporter is an evolutionarily conserved calcium channel, and its biophysical properties and relevance to cell death, bioenergetics and signalling have been investigated for decades. However, the genes encoding this channel have only recently been discovered, opening up a new ‘molecular era’ in the study of its biology. We now know that the uniporter is not a single protein but rather a macromolecular complex consisting of pore-forming and regulatory subunits. We review recent studies that harnessed the power of molecular biology and genetics to characterize the mechanism of action of the uniporter, its evolution and its contribution to physiology and human disease.

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Dairy products, calcium, and prostate cancer risk in the Physicians’ Health Study

By | Research Study

Dairy products, calcium, and prostate cancer risk in the Physicians’ Health Study

June M Chan, Meir J Stampfer, Jing Ma, Peter H Gann, J Michael Gaziano, and Edward L Giovannucci

Background: A high calcium intake, mainly from dairy products, may increase prostate cancer risk by lowering concentrations of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], a hormone thought to protect against prostate cancer. The results of epidemiologic studies of this hypothesis are inconclusive.

Objective: We investigated the association between dairy product and calcium intakes and prostate cancer risk in the Physicians’ Health Study, a cohort of male US physicians.

Design: At baseline, the men answered abbreviated dietary questionnaires. During 11 y of follow-up, we documented 1012 incident cases of prostate cancer among 20885 men. We estimated dairy calcium intake on the basis of consumption of 5 major dairy products and used logistic regression to estimate relative risk.

Results: At baseline, men who consumed >600 mg Ca/d from skim milk had lower plasma 1,25(OH)2D3 concentrations than did those consuming ≤150 mg Ca/d [71 compared with 85 pmol/L (30.06 compared with 35.64 pg/mL); P = 0.005]. Compared with men consuming ≤ 0.5 daily servings of dairy products, those consuming >2.5 servings had a multivariate relative risk of prostate cancer of 1.34 (95% CI: 1.04, 1.71) after adjustment for baseline age, body mass index, smoking, exercise, and randomized treatment assignment in the original placebo-controlled trial. Com- pared with men consuming ≤150 mg Ca/d from dairy products, men consuming >600 mg/d had a 32% higher risk of prostate cancer (95% CI: 1.08, 1.63).
Conclusions: These results support the hypothesis that dairy products and calcium are associated with a greater risk of prostate cancer.

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Vitamin D, calcium, and retinol intake, and pancreatic cancer

By | Research Study

Vitamin D, calcium, and retinol intake, and pancreatic cancer

Lydia B. Zablotska • Zhihong Gong • Furong Wang • Elizabeth A. Holly • Paige M. Bracci

Objective: The aim of this study was to evaluate a com- plex association among intake of dietary vitamin D, cal- cium, and retinol, and pancreatic cancer risk.
Methods Pancreatic cancer cases (n = 532) diagnosed in 1995–1999 were identified using rapid case ascertainment methods and were frequency matched to population-based controls (n = 1,701) in the San Francisco Bay Area. Detailed dietary data were collected during in-person interviews using a validated semi-quantitative food- frequency questionnaire. Adjusted unconditional logistic regression was used to estimate odds ratios (ORs) and confidence intervals.

Results: In men, increased pancreatic cancer risk was associated with currently recommended dietary vitamin D intake levels (highest (C450 IU/day) vs. lowest (\150 IU/ day) intake, OR = 2.6, trend-p = 0.009) and total vitamin D intake from diet and supplements (for \800 IU/day). ORs for dietary vitamin D intake remained increased after adjustment for intake of retinol and calcium, although confidence intervals included unity. Stratified analyses showed that ORs were higher among men with lower intake of retinol and lower physical activity but there was no evidence of statistical interaction. No associations with vitamin D intake were observed among women, although ORs typically were elevated. ORs increased with increased dietary calcium intake among men (trend-p = 0.008) and not women.

Conclusions: Our results among men showing an increased risk of pancreatic cancer associated with dietary intake of vitamin D and of calcium require confirmation in further studies. Continued investigation is needed to clarify the complex role of vitamin D and calcium in pancreatic cancer risk and to determine their optimal intake level and preventive effects for pancreatic cancer.

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Calcium, Magnesium, and Colorectal Cancer

By | Research Study

Calcium, Magnesium, and Colorectal Cancer

Qi Dai1, Robert S. Sandler2, Elizabeth L. Barry3, Robert W. Summers4, Maria V. Grau5, and
John A. Baron2,5,6

High calcium consumption may confer a reduced risk of colorectal cancer.1,2 Dai and colleagues3 recently reported in a case-control study that intake of calcium may be associated with a decreased risk of colorectal adenoma only when the dietary calcium:magnesium intake ratio is low. This finding provides one possible interpretation for inconsistencies in previous studies of the association of calcium intake with risk of colorectal neoplasia.4

Belonging to the same family in the periodic table, calcium (Ca2+) and magnesium (Mg2+) share the same homeostatic control system and have the potential to antagonize each other physiologically.5 A high calcium intake reduces absorption of both magnesium and calcium,6 whereas moderate magnesium deprivation results in negative magnesium balance but increased calcium retention7. Due to the potential competition between magnesium and calcium, we hypothesized that the dietary calcium:magnesium ratio may modify the effects of calcium supplementation on colorectal carcinogenesis.

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Calcium, cancer and killing

By | Research Study

Calcium, cancer and killing: The role of calcium in killing cancer cells by cytotoxic T lymphocytes and natural killer cells

Eva C. Schwarz, Bin Qu, Markus Hoth:

Killing cancer cells by cytotoxic T lymphocytes (CTL) and by natural killer (NK) cells is of vital importance. Cancer cell proliferation and apoptosis depend on the intracellular Ca2+ concentration, and the expression of numerous ion channels with the ability to control intracellular Ca2+ concentrations has been correlated with cancer. A rise of intracellular Ca2+ concentrations is also required for efficient CTL and NK cell function and thus for killing their targets, in this case cancer cells. Here, we review the data on Ca2+-dependent killing of cancer cells by CTL and NK cells. In addition, we discuss emerging ideas and present a model how Ca2+ may be used by CTL and NK cells to optimize their cancer cell killing efficiency. This article is part of a Special Issue entitled: 12th European Symposium on Calcium.

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Regulation of calcium signaling in lung cancer

By | Cancer

Regulation of calcium signaling in lung cancer

Haihong Yang, Qi Zhang, Jianxing He, Wenju Lu

Lung cancer is the most common malignant tumor in the world. Calcium is a ubiquitous cellular signal, which is crucial in cancer. This re- view presents regulation of calcium signaling in lung cancer. Altered expression of specific Ca2+ channels and Ca2+-binding proteins are characterizing features of lung cancer, which regulate cell signaling pathway leading to cell proliferation or apoptosis. Chemoresistance is frequent in lung cancer. Altered endoplasmic reticulum Ca2+ homeostasis of lung cancer cell is correlated with drug resistance. Hypoxia has a vital role in tumor angiogenesis, metastasis, apoptosis. And Ca2+ channels are open induced by hypoxia with the increase of Ca2+ influx causing tumor growth.

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Where cancer meets calcium — p53 crosstalk with EF-hands

By | Research Study

Where cancer meets calcium — p53 crosstalk with EF-hands

Mitsuhiko Ikura and Kyoko L. Yap:

S100B, an EF-hand Ca2+-binding protein, grasps the C-terminus of the tumor suppressor p53 and inhibits protein kinase C-dependent phosphorylation and acetylation of p53 in a Ca2+-dependent manner. The mode of interaction between S100B and p53 is different from the interactions seen in S100A–annexin complex structures.

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Switching p53 states by Calcium: Dynamics and Interaction of Stress Systems

By | Research Study

Effects of Sigma Anti-bonding Molecule Calcium Carbonate on bone turnover and calcium balance in ovariectomized rats

Md. Jahoor Alam,a Gurumayum Reenaroy Devi,a Ravins,a Romana Ishrat,a Subhash M. Agarwalb and R. K. Brojen Singh*a

The integration of calcium and a p53–Mdm2 oscillator model is studied using a deterministic as well as a stochastic approach, to investigate the impact of a calcium wave on single cell dynamics and on the inter-oscillator interaction. The high dose of calcium in the system activates the nitric oxide synthase, synthesizing nitric oxide which then downregulates Mdm2 and influences drastically the p53–Mdm2 network regulation, lifting the system from a normal to a stressed state. The increase in calcium level switches the system to different states, as identified by the different behaviours of the p53 temporal dynamics, i.e. oscillation death to sustain the oscillation state via a mixed state of dampened and oscillation death states. Further increase of the calcium dose in the system switches the system from sustained to oscillation death state again, while an excess of calcium shifts the cell to an apoptotic state. Another important property of the calcium ion is its ability to behave as a synchronizing agent among the interacting systems. The time evolution of the p53 dynamics of the two diffusively coupled systems at stress condition via Ca2+ shows synchronization between the two systems. The noise contained in the system interestingly helps the system to maintain its stabilized state (normal condition). However, noise has the tendency to destruct the synchronization effect, which means that it tries to restrict the system from external signals to maintain its normal condition. However, at the stress condition, the synchronization rate is found to be faster.

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SAC and Cancer Therapy

By | Resources, SAC

SAC Therapy for Cancer Treatment

Cellular and Systemic Restoration Build Uninhabitable Environment for Cancer

SAC in a
Nutshell

SAC (Sigma Antibonding Calcium Carbonate) is the only true ionic calcium delivery system that provides calcium in free ionic state, which is the only physiologically active form of calcium in our body. Normally, calcium from diet and supplements enters our body in the protein-bound form and therefore, cannot trigger the same physiological responses as SAC. Resolving calcium deficiency better than protein-bound calcium, SAC triggers ionic-calcium-sensitive physiological responses that counteract the root cause of diseases and brings natural healing reactions of our body from cellular to the systemic level.

Cancer is a genetic disease—that is, it is caused by changes to genes that control the way our cells function, especially how they grow and divide and thus multiply without stopping and spread into surrounding tissues. Cancer can start almost anywhere in the human body.

Normally, human cells grow and divide to form new cells as the body needs them. When cells grow old or become damaged, they die, and new cells take their place. When cancer develops, however, this orderly process breaks down, and damaged cells survive when they should die and new cells form when they are not needed. These extra cells can divide without stopping and may form growths called tumors. Cancers of the blood, such as leukemia, generally do not form solid tumors.

Cancerous tumors are malignant and invade nearby tissues. Also, some cancer cells can break off and travel to distant places in the body through the blood or the lymph system and form new tumors far from the original tumor.

How Does Cancer Grow and Spread?

How Cancer Cells Grow Uncontrollably

Cancer cells grow out of control and become invasive because they are able to ignore signals that normally tell cells to stop dividing or begin a process known as programmed cell death, or apoptosis, which the body uses to get rid of unneeded cells.

Also, cancer cells are also often able to evade and hide from the immune system that protects the body from infections and other conditions. Tumors can also use the immune system to stay alive and grow. Moreover, cancer cells may be able to influence the normal cells, molecules, and blood vessels that surround and feed a tumor—an area known as the microenvironment.

How Cancer Arises and Spreads

Each person’s cancer has a unique combination of genetic changes. As the cancer continues to grow, additional changes will occur even within the same tumor.

The genetic changes that contribute to cancer tend to affect three main types of genes – proto-oncogenes, tumor suppressor genes, and DNA repair genes. All these genes are involved in normal cell growth, maintenance, and division.

Before cancer cells form in tissues of the body, the cells go through abnormal changes called hyperplasia and dysplasia. In hyperplasia, there is an increase in the number of cells in an organ or tissue that appear normal under a microscope. In dysplasia, the cells look abnormal under a microscope but are not cancer. Hyperplasia and dysplasia may or may not become cancer.

A cancer that has spread from the place where it first started to another place in the body is called metastatic cancer .

In metastasis, cancer cells break away from where they first formed (primary cancer), travel through the blood or lymph system, and form new tumors (metastatic tumors) in other parts of the body.

The metastatic tumor is the same type of cancer as the primary tumor. Metastatic tumors can cause severe damage to how the body functions, and most people who die of cancer die of metastatic disease.

Common Types of Cancer

There are more than 100 types of cancer. Types of cancer are usually named for the organs or tissues where the cancers form. Cancers also may be described by the type of cell that formed them, such as an epithelial cell or a squamous cell. Here are some categories of cancers that begin in specific types of cells:

Melanoma is cancer that begins in cells that become melanocytes, which are specialized cells that make melanin (the pigment that gives skin its color). Most melanomas form on the skin, but melanomas can also form in other pigmented tissues, such as the eye.

Sarcomas are cancers that form in bone and soft tissues, including muscle, fat, blood vessels, lymph vessels, and fibrous tissue (such as tendons and ligaments). Osteosarcoma is the most common cancer of bone.

Lymphoma is cancer that begins in lymphocytes (T cells or B cells). These are disease-fighting white blood cells that are part of the immune system. In lymphoma, abnormal lymphocytes build up in lymph nodes and lymph vessels, as well as in other organs of the body.

Carcinomas are the most common type of cancer. They are formed by epithelial cells, which are the cells that cover the inside and outside surfaces of the body. There are many types of epithelial cells, which often have a column-like shape when viewed under a microscope.

Multiple myeloma is cancer that begins in plasma cells, another type of immune cell. The abnormal plasma cells, called myeloma cells, build up in the bone marrow and form tumors in bones all through the body.

Cancers that begin in the blood-forming tissue of the bone marrow are called leukemia. These cancers do not form solid tumors. Instead, large numbers of abnormal white blood cells build up in the blood and bone marrow, crowding out normal blood cells. The low level of normal blood cells can make it harder for the body to get oxygen to its tissues, control bleeding, or fight infections.

Types of Treatment for Cancer

There are many types of conventional cancer treatment. The types of treatment that you receive will depend on the type of cancer you have and how advanced it is. The main types of cancer treatment include:

  • Surgery
  • Radiation Therapy
  • Chemotherapy
  • Immunotherapy
  • Targeted Therapy
  • Hormone Therapy
  • Stem Cell Transplant
  • Precision Medicine

Radiation Therapy

At high doses, radiation kills cancer cells or slows their growth. Radiation therapy is used to either treat cancer or ease cancer symptoms.

Radiation therapy does not kill cancer cells right away. It takes days or weeks of treatment before cancer cells start to die. Then, cancer cells keep dying for weeks or months after radiation therapy ends.

There are two main types of radiation therapy, External Beam Radiation Therapy and Internal Radiation Therapy.

Internal Radiation Therapy

Internal radiation therapy is a treatment in which a source of radiation is put inside your body. The radiation source can be solid or liquid in the form of seeds, ribbons, or capsules placed in your body in or near the cancer. You receive liquid radiation through an IV line. Liquid radiation travels throughout your body, seeking out and killing cancer cells.

External Beam Radiation Therapy

External beam radiation therapy comes from a machine that aims radiation at your cancer and treats a specific part of your body.

Radiation Therapy Side Effects

Radiation not only kills or slows the growth of cancer cells, it can also affect nearby healthy cells. Damage to healthy cells can cause side effects. The most common side effect of radiation therapy is fatigue, which is feeling exhausted and worn out. Fatigue can happen all at once or little by little. Healthy cells that are damaged during radiation treatment almost always recover after it is over. But sometimes people may have side effects that are severe or do not improve. Other side effects may show up months or years after radiation therapy is over. Doctors try to protect healthy cells during treatment by using as low a dose of radiation as possible, by spreading out treatment over time, and by aiming radiation at a precise part of your body.

Chemotherapy

Chemotherapy is used to treat many types of cancer. For some people, chemotherapy may be the only treatment you receive. But most often, you will have chemotherapy and other cancer treatments. The types of treatment that you need depends on the type of cancer you have, if it has spread and where, and if you have other health problems.

When used with other treatments, chemotherapy can:

Make a tumor smaller before surgery or radiation therapy, and destroy cancer cells that may remain after treatment with surgery or radiation therapy. Chemotherapy can also help other treatments work better.

Chemotherapy Side Effects

Chemotherapy not only kills fast-growing cancer cells, but also kills or slows the growth of healthy cells that grow and divide quickly. Examples are cells that line your mouth and intestines and those that cause your hair to grow. Damage to healthy cells may cause side effects, such as mouth sores, nausea, and hair loss. Side effects often get better or go away after you have finished chemotherapy. The most common side effect is fatigue, which is feeling exhausted and worn out.

Immunotherapy

One reason that cancer cells thrive is because they are able to hide from your immune system. Certain immunotherapies can mark cancer cells so it is easier for the immune system to find and destroy them. Other immunotherapies boost your immune system to work better against cancer.

Immunotherapy is a type of cancer treatment that helps your immune system fight cancer. The immune system is made up of white blood cells and organs and tissues of the lymph system. Many different types of immunotherapy are used to treat cancer. They include:

Monoclonal Antibodies

These are drugs that are designed to bind to specific targets in the body and cause an immune response that destroys cancer cells. Other types of monoclonal antibodies can “mark” cancer cells so it is easier for the immune system to find and destroy them. These types of monoclonal antibodies may also be referred to as targeted therapy.

Adoptive Cell Transfer

This is a treatment that attempts to boost the natural ability of your T-cells to fight cancer. T-cells are a type of white blood cell and part of the immune system. Researchers isolate T-cells that are most active against your cancer from the tumor and then grow large batches of these T-cells in the lab and then inject them via a needle in your vein.

Cytokines

These are proteins that are made by your body’s cells. They play important roles in the body’s normal immune responses and also in the immune system’s ability to respond to cancer.

Treatment Vaccines

These work against cancer by boosting your immune system’s response to cancer cells.

BCG

This is an immunotherapy that is used to treat bladder cancer. When weakened form of the bacteria that causes tuberculosis is inserted directly into the bladder with a catheter, BCG causes an immune response against cancer cells.

Immunotherapy Side Effects

Immunotherapy can cause side effects. The side effects you may have depend on the type of immunotherapy you receive and how your body reacts to it. The most common side effects are skin reactions at the needle site. These side effects include pain, swelling, soreness, redness, rash, fever, chills, nausea, fatigue, etc.

SAC Ionic Calcium Therapy for Cancer

Ionic Calcium Repairs Cellular Kill Switch (P53 Gene)

Innovative ionized calcium therapy destroys cancer cells by re-activating muted gene responsible for apoptosis or cell self-destruction. In normal cells, P53 gene triggers cell death when a cell is damaged or aging, allowing new healthy cells to replace it.

However, many cancer cells mass produce NF-kB protein which interferes with the function of P53 gene and turns off apoptosis, causing damaged cells to continue dividing and multiplying. NF-kB is produced in cytoplasm, and then translocated into the nucleus and binds to P53 gene, inhibiting its original functions.

Calcium ions, once introduced into cancer cells, inhibits NF-kB’s effect on P53 gene and therefore restores the function of self-destruction. Cancer cells have only 1% of calcium ions of healthy normal cells, linking widespread calcium deficiency in America to rise of occurrence of cancer for further studies.

In the advanced stage of cancer where P53 gene is damaged beyond repair, calcium ions block lactic acid and inhibit the inflow of glucose into the cells, causing cancer to starve to death.

Ionic Calcium Restores RAS Inhibitor Gene Mutation

Calcium ions fight cancer in many other way as well. In pancreatic cancer (90%), intestine cancer, lung cancer and thyroid cancer (50%), liver cancer (30%), and leukemia (30%), RAS inhibitor gene mutation in cancer can be found and Ca2+ corrects cancer suppressor gene to normal.

Ionic Calcium Inhibits Inflammatory Enzymes that Fuel Cancer Growth and Metastasis

In brain tumor, intestine cancer, pancreatic cancer, breast cancer, bladder cancer, lung cancer, and more, cyclooxygenase-2 enzyme (COX-2) is responsible for spreading cancer. Calcium ions inhibit this enzyme by making body fluid alkaline. After all, calcium is our body’s natural acid buffer.

Dietary acids affect your body’s buffering capability, which may cause a calcium loss from your bones to counteract the acidity. Acidosis is caused by kidney disease, dehydration, alcohol, high dietary protein, and other health problems. Increased cancer risk is also associated with dietary lifestyles that alter systemic acid-base balance over time and lead to a sub-clinical or low-grade state of metabolic acidosis. The relationship between diet and cancer risk prompts questions about the role of acidosis in the initiation and progression of cancer. SAC counteracts acidosis.  Due to 60 mV flowing in our body, calcium also exists in our body in a free ionic state which forms Ca(OH)2, inducing alkalinity.

Ionic Calcium Restores Ideal pH for Optimum Oxygen Delivery

Ionic Calcium Switches On Immune Response Around Acidic Tumor Environment

In battling cancer, it is important to eliminate the environment that first caused or nurtured cancer cells. Studies have shown that blood oxygen level of patients with cancer is much lower than that of healthy people. Also, a Nobel prize winner (Otto Warburg, 1883-1970) found that depriving a cell 35% of its oxygen for 48 hours made it cancerous.

There is close relationship between lack of both oxygen and calcium in cancer cells because calcium is responsible for delivering oxygen to intercellular space. Therefore, lack of calcium in cancer cells leads to lack of oxygen which leads to highly acidic environment which cancer favors.

Cancer cells upregulates metabolism to aerobic glycolysis (fermentation) in which glucose accumulates as lactic acid making cancer tumor environment highly acidic which provides cancer with ideal thriving environment in which to grow and to spread. By making intercellular space reach ideal pH by calcium ions, more influx of oxygen with calcium ions will eliminate cancer-thriving environment.  Raising pH around the tumor also turns on strong immune response, exposing cancer from hiding.

SAC Therapy Aids Oxygenation

Unlike normal cells, cancer cells metastasize uncontrollably in oxygen-deprived environments due to their ability to maintain and perform cellular functions via anaerobic metabolisms such as angiogenesis, apoptosis resistance, and proliferation which all aids cancer survival.  On the contrary, good oxygenation may help to reverse such a cancer favorable environment.  However, for cancer patients, good oxygenation is hampered because of the acidic environment created by cancer.

A Role of PTHrP in Reducing Oxygenation of Cells

Cancer cells occasionally secrete a parathyroid hormone-related protein (PTHrP) which is often used in cancer diagnosis as the first sign of malignancy. PTHrP plays a significant role in cancer initiation, growth, and metastasis. Moreover, it is believed that the function of PTHrP is similar to that of parathyroid hormone (PTH) in bone regulation, considering that both PTHrP and PTH stimulate the release of calcium in the bones to increase the concentration of blood-calcium concentration, and are capable of inducing hypercalcemia and osteoporosis by triggering calcium resorption from bones.

When ionic calcium concentration increases as a result of this uncontrolled calcium secretion from bones caused by PTHrP, the blood pH decreases due to phosphorus release, making the body more acidic.  Importantly, as motioned above, as the blood pH becomes acidic, the hemoglobin and oxygen-binding affinity decreases, resulting in a shortage of oxygen supply to tissues and organs. Hence, a reduction in the oxygen supply may foster cancer proliferation and metastasis.

Similarly, the binding affinity between calcium and its transporter protein depends heavily on the blood pH. In a low pH environment, the binding affinity decreases, leading to an increase in the concentration of blood-circulating ionized calcium. In contrast, in a high pH environment, calcium tends to tightly bind to protein, resulting in a reduction in free ionized calcium levels. Therefore, it has been proposed that an increased fraction of free ionized calcium due to pH fluctuation could be the body’s innate response to neutralize acidity!  Now, SAC therapy can provide enough ionic calcium to raise pH and increase oxygenation. 

Ionic calcium itself does not promote acidity in the blood but rather used as a neutralizing factor in fluctuating pH. Hence ionic calcium therapy may play significant roles in 1) preventing osteoporosis caused by excessive PTHrP secretion, and 2) neutralizing the blood pH and thereby promoting hemoglobin-oxygen affinity for enhanced oxygen supply.

To draw the effect of SAC on the restoration of oxygen supply, we emphasize its outcome on the systemic regulation of hormones associated with calcium homeostasis. It is widely known that significant regulation of the calcium homeostasis, and therefore of bone metabolism, is regulated by the secretion of PTH and thyroid hormone (TH). SAC initiates systemic regulation of both PTH and TH involved in calcium homeostasis, which, in turn, stabilizes the absorption and resorption of bones. Therefore, the systemic control of thyroid hormones by SAC can be efficiently used to normalize the blood pH level caused by excessive ionized calcium circulation in the blood mediated by PTHrP secreted from cancer cells. Hence taking into account several studies that demonstrated the relationship between calcium regulation in cancer and blood pH levels, the following graph depicts the general idea of oxygenation and calcium regulation (Figure 1).

Road to Recovery under SAC Therapy

SAC rebuilds underlying health while liberating from many symptoms

(Phases 1 to 3 are based on many successful SAC therapy as either stand alone or adjunct therapy alongside alternative treatment.  Individual results vary. Below does not apply as adjunct or adjuvant therapy for conventional cancer treatments.)

Critical Minimum 3 Months of SAC Treatment

Because of SAC’s beneficial impact at systemic levels, the health improvement experienced may fluctuate for the first 3-month period of the treatment for most patients until these positive results start to dominate during the second 3-month period of therapy.

It is also during the first 3-month period of the treatment when healing reactions are experienced for some patients as our body tries to balance the healing effects of SAC.  Healing reactions from SAC tends to subside and disappear for most people during the second 3-months period of SAC therapy.

Therefore, it is advised that SAC treatment should be given at least for a minimum of 3-month of the period to look for any positive results before committing another 3-month of SAC therapy.  If any positive results are observed during the first 3-month period, then we recommend another 3-month period of therapy to be given to curb the disease momentum.

During these two 3-month periods, many patients experienced enough positive effects of SAC therapy that encourages them to continue the treatment all the way to full recovery.

EXAMPLE: CASE STUDY OF A CANCER PATIENT 

In the case of an 80-year old lady with last stage multiple myeloma, the road to her full recovery had mixed signals.

In the first 3-months of SAC therapy, there was a clear sign that the therapy was working, followed by a dip that seems to indicate that it was not. Because there were some good signs, the second 3-months of treatment were given, which also had ups and downs. However, during the second 3-months period of treatment, there were more positive effects, including her general feeling of wellbeing.

When Molly had committed to the third 3-months of treatment, her lab results and health steadily climbed up to full cancer remission. Her bone density returned to normal (-0.9) from osteoporosis (-3.4), and the kidney function bounced back up to GFR 60 from GFR 10.

Sample Cancer Clinical Cases

Molly started on Maragen when she stopped taking anticancer medication for her last stage multiple myeloma. After taking Maragen for ten months, her blood test results indicated almost normal. Molly had other benefits from taking SAC treatment. Her rib fracture healed up quickly and even low kidney function improved significantly, and recovered from thrombosis. From osteoporosis, her bone density jumped up to normal range for her age, and she is still cancer free to this date. (2017)When asked, if she felt healthier and stronger after taking Maragen, her reply was a positive “Maybe,” and with a big smile she added, “Thank you very much for giving me Maragen.”

CANCER SAMPLE CASE #3

Clinical Example: Yongho Kim (male, 80 years old) was diagnosed with the last stage of leukemia (2013). Upon receiving the SAC therapy for 5 months (12 bottles of MaraGen), his cancer is in remission. The chart below shows noticeable changes in the blood test results within one month. He is fully recovered and healthy as of today (Feb. 2017). Click on the chart to see the full result.

Lactic Acid Reduction Clinical Trial

(Important Indicator for Effectiveness as a Cancer Treatment)

Twenty swimmers from Korea University swim team taking two doses a day for 14 days proved that SAC neutralizes lactic acid effectively. They all broke their swimming records as a result.

Lactic acid, which is the byproduct of carcinoma cancer cells, is known to inhibit the immune functions around cancer tumors. Neutralizing the lactic acid and bringing pH back to a normal level is crucial in cancer treatment.

A Doctor’s Anecdotal Note of Her Patient: A 35-year-old man with abdominal bloating x 1/12. He underwent an abdominal CT scan to find a 30cm solid tumor retroperitoneally. Compression of the left ureters and hydronephrosis of the left kidney and multiple abdominal and pelvic nodes. He also had ascites. The biopsy showed Hodgkin’s Lymphoma. The Patient underwent 3 cycles of chemo from March till May of 2018 (palliative care) as the patient was very ill. He also received concurrent IV vitamin C (60-90 grams) twice a week. In May he received oral Maragen (twice daily), continued one more cycle of chemo and he was very well. The tumor shrunk to non-detectable size and in June 2018, he received 2 doses of IV Maragen also whilst consuming oral Maragen. His tumor is not seen by ultrasound and there are no ascites. The patient is very well and his blood parameters have returned to normal. (Full medical papers available)

Antonius R., Male, 35, Malaysia

Personal Testimony: I am a US Air Force pilot and one day got diagnosed with a brain tumor the size of a golf ball. I was devastated. However, when I heard about MaraGen, I took it for three months before I got another brain scan at the Washington Bethesda Medical center. The doctor asked me incredulously, “How come I do not see the golf ball sized tumor?” MaraGen is a miracle supplement!

Merry – Age 30, Female

Personal Testimony: In March of 2010, at the age of 60, I was diagnosed with prostate cancer, which had spread to the large colon. I had to remove the tumor from it surgically. One day in early July, I wrote up my last will for my children and family. I also stopped by Dr. Lee’s lab to bid farewell as we have been friends for a while, and he introduced me to SAC and recommended me to take it. I did not know what it was, but I brought it home and took it as instructed. Up until that moment, because of prostate cancer, I couldn’t urinate properly as it made urination very uncomfortable and difficult. But after taking SAC, I was able to urinate as if I was free from prostate cancer. Dr. Lee encouraged me to take SAC 3-4 times daily. A month later, to my disbelief, my blood test result indicated that my PSA levels dropped from 28 to 4, and I also felt great physically.

On August 20, I was getting ready to have ostomy surgery. Following my doctor’s recommendation, I could not help it though the thought of it made me cringe. Arriving at the hospital on the day of surgery, Dr. Lee was there to pray for me. He left as I was escorted into the operation room for the procedure. However, when I woke up from anesthesia and realized that I had no ostomy bag on my belly, I was confused. I asked if the operation had not started yet or if there were any complications. The surgeon replied that there was no need to remove the rectum! Hallelujah! I could not believe my ears and immediately informed Dr. Lee, who was as happy as I was. In January 2012, I had another blood test, and the result came out normal with no traces of cancer. It felt like a dream. I am so thankful for Dr. Lee’s development of SAC.

In January 2020, I am still healthy with no cancer!”

Mr. Cho, Male, 68, Surrey, BC, Canada

SAC may trigger the increase of cancer markers:

As tumors are subsiding by SAC therapy (apoptosis and other anti-cancer effects), marker proteins are dumped into blood vessels and may reflect increased level in blood works. For cancer patients whose health is deteriorating, increasing cancer marker comes with deteriorating blood work. However, if patients receiving SAC therapy see improving blood works with incresed energy and still see an abrupt increase of cancer markers, then most likely it is because the cancer is receding rapidly and therefore close follow up of doctors is advised.

SAC may cause the increase of tumor size:

Due to rapid death of cancer cells, tumors could internally fill with fluids and may look bloated and larger in the scans. This is not to be mistaken as tumor growth. Tumor density test may reveal decreased tumor density despite the increase of the size. Patients are advised to confirm with tumor density test.

Ionic Calcium Targets Cancer Both Within and Without

When popular therapies focus on destroying cancer cells from outside only, ionic calcium therapy targets cancer cells both within and without by restoring our body’s natural process for eliminating damaged cells and by eliminating the environment cancer favors, all naturally.

Pronuvia’s new innovative SAC transport system delivers calcium ions directly into our blood vessels and carries it to every parts of our body affected by cancer, even where chemotherapy has difficulty reaching.

The invention of New Calcium Carbonate

Sigma Anti-bonding Calcium Carbonate (SAC-CaCO3)

SAC is the world’s first calcium-ion-delivery-system, which safely and effectively elevates the level of calcium-ion concentration in our blood. By utilizing a very weak chemical bonding, namely sigma antibonding, to calcium carbonate molecules, Calcium & Bone Health Institute of Canada (CBHI) invented new calcium carbonate, which maintains loosely held calcium ion to its carbonate group. The antibonding makes the molecules exhibit electrical charge and attract water molecules via hydrogen bonding. Making sigma antibonding stable at room temperature was, in itself, a technological breakthrough after ten years of R&D.

Because of the weak chemical bonding of SAC, calcium ion is easily detached and passively absorbed into our system through stomach lining as ions via diffusion and osmotic pressure, not requiring digestion, vitamin D, nor peptides for absorption.  This is called passive transport.

Because of our body’s natural sensitivity to fluctuations of serum plasma ionic calcium level, a minimal elevation of ionic calcium concentration achieved by SAC can trigger hormonal responses, such as the release of TSH and calcitonin to trigger bone-building osteoblasts. SAC therapy utilizes ionic calcium as a signaling agent to trigger our body’s self-healing responses to reverse calcification from cellular to systemic level, causing domino effects of healing processes to rebuild our health. SAC’s healing pathway is genuinely unique.

"SAC is the world's first safe calcium-ion delivery system administered directly into our body in oral form. SAC triggers healing mechanisms no other calcium supplementation can achieve"

What is Sigma Anti-bonding?

Anti-bonding orbitals are essentially the “opposite” of bonding orbitals. They are formed when atomic orbitals combine in ways that lead to predominantly destructive interference.

 

The key feature of anti-bonding orbitals is that the molecular orbitals have a higher energy than the corresponding atomic orbitals. Thus, the molecule has a higher energy than the separated atoms (atoms separated by a large distance) and the atoms would prefer to be in the lower atomic state.

Anytime two atomic orbitals combine to give a lower-energy bonding orbital, an analogous higher energy anti-bonding orbital is also formed. Above is a figure depicting this simple bond/anti-bond molecular orbital diagram that we had for hydrogen. The diagram also shows that electrons (in this case) completely fill the bonding orbital and leave the anti-bonding orbital empty.

 

SAC calcium’s sigma anti-bonding keeps the calcium atoms in the molecule very loose and unstable. Our innovative technology locks calcium atoms in place until it is released in our body.

SAC Calcium is an innovative new bio-material based on calcium carbonate derived from small oyster shells. Highly advanced process formulates it to maintain positive charges (2+) by altering the bonding structure, namely sigma anti-bonding. The positive charges of the molecule attract water molecules to cluster around it, making it incredibly water soluble and allowing direct and passive absorption into the body.

SAC Calcium is directly absorbed because of its anti-bonding positive charge, and is immediately bio-available. SAC bypasses active transport delivery that requires digestion with peptides and vitamin D, a complicated process that leaves absorbed calcium far less bio-available.

Unique Physical Properties of SAC Calcium

Because of electrical charge of SAC calcium cabonate molecule that interacts with hydrogen bonding of water molecules, an open bottle of SAC evaporates calcium together with water molecules and crystalize with CO2 in the air.

SAC is 200x more soluable in water compared to calcium carbonate and 3x more reactive in chemical reaction.

Unique Passive Absorption of SAC Calcium

Active Transport of Regular Calcium

Regular calcium intake from diet or supplements need strong stomach acid with peptides and vitamin D3 to digest and be absorbed in small intestines as protein-bound calcium, which is not readily utilized with aging because of sedentary lifestyle, hormonal changes, and poor diet.  Various side effects of inactive calcium include kidney stones, blood vessel calcification, stroke, heart attack, etc.  Most, if not all, calcium supplements fall in this category and may aggrevate body-wide calcification which is known to be one of many major cause of degenerative diseases.

Passive Transport of SAC Calcium

Unlike regular calcium intake, no Vitamin D3 and peptides are needed for absorption. SAC diffuses passively through digestive tract cell linings (mucosa) as ions, not requiring physiological energy from our body and readily available for immediate use.

Ionic calcium is immediately utilized to bring calcium homeostasis :

  • Corrects calcium signaling
  • Reduces cellular oxidative stress
  • Restores mitochondrial function
  • Triggers decalcification

SAC is Absorbed through Diffusion & Osmotic Pressure

About 5 to 7.6 mg of SAC solution is mixed with 500ml of water for optimun absorption as ionic calcium.  SAC is takin in an empty stomach and is completely absorbed within 30 minutes through diffusion and osmotic pressure. Although it is in a small intake amount, slight elevation of ionic calcium in the blood is enough to trigger TSH (thyroid stimulating hormone) to initiate strong bone-building osteoblasts.  SAC’s cascading effects on physiological functions of our body also activate inactive protein-bound calcium in our blood to further boost and maintain good bone health.

SAC is the first calcium-ion-delivery-system to help build and support strong bones while promoting normal bone mass. SAC also plays an important role in other functions such as nerve transmission and muscular function. Unlike other electrically neutral calcium supplements that require Active Transport, SAC calcium’s Passive Transport brings full absorption of calcium without the need for Vitamin D, peptide, or other agents.

Inside our cells, SAC calcium quickly breaks down to yield calcium ions, which are absorbed into capillaries and triggers our body’s natural bone formation process.

Physiological Effects of SAC

After intake, SAC’s effect lasts about four hours in our body, initially raising the serum ionic calcium concentration to a higher yet safe level to trigger various physiological functions before bringing down the serum ionic calcium concentration down to the average physiological level.

While ionic calcium level is elevated, bone-building osteoblast with osteoclastic activity is triggered to raise the bone turnover rate, repairing and rebuilding bones. This process also activates idle protein-bound calcium, releasing both ionic calcium and protein, further fueling bone-building and clearing body-wide calcification. Ionic calcium also aids cellular metabolism, releasing more ATP (adenosine triphosphate) and raising body temperature. As kidneys try to excrete excess ionic calcium through urination, an urge to urinate within an hour of taking SAC is experienced, which is both healthy and normal, indicating that SAC is working.

STRONGER BONES LEAD TO A HEALTHIER LIFE

A long term, follow up study done in Denmark for 35,000 people revealed that the people with strong bones in their 50’s lived 11.6 years longer.
YET, in Canada, 49% of infants are born with calcium deficiency. Only 70% recover after breastfeeding. Calcium deficiency during pregnancy and infancy leads to serious health issues.

Bone Loss Leads to 150+ Degenerative Diseases

Bone health is directly related to our overall health. Emptier bone characterized by osteoporosis or osteopenia indicates not only a higher risk of fracture but also a greater chance of developing degenerative diseases. Why? Because emptying bones cause calcification in both cellular and systemic levels, causing cellular communications mayhem by disrupting calcium signaling.

Promising Animal Clinical Trials

Calcium ions in the blood are so vital that the body cannot permit it to fluctuate. Therefore, even a slight increase in the concentration of ionized calcium in the blood triggers the bone building process to take excess calcium into bones. Utilizing this process is by far the most effective and safe way to support strong bones since it follows the body’s natural bone building mechanism. This amazing effect of SAC was observed in this animal clinical trial through the bone break test where SAC ‘treated’ bone displayed almost 100x bone building power compared to regular calcium carbonate.

Importance of SAC therapy goes beyond the stronger bone-building.  Although most of our body’s calcium is stored in bone, the tiny amount that circulates in your bloodstream is disproportionately vital to good health. About half of this circulating serum calcium (50%) is “ionized”, which means it carries electrical charges and this calcium ions (Ca2+) are the only physiologically active form that can be recognized by our body and responsible for numerous functions of our body such as the firing of muscle and nerve cells, promoting blood clotting, preventing the depletion of bone mass, securing proper cellular functions by preserving calcium signaling, etc.  As we age, this vital ionic calcium homeostasis is disrupted as our bone breaks down and calcifies trillions of cells.  SAC therapy can restore this fragile calcium homeostasis and gives our body a chance to fight back the onset of 150+ degenerative diseases  that are thought to be caused by calcium displacement.

Osteoporosis Reversed under SAC Therapy

(Lab Anim Res 2011: 27(4), 301-307, 2011)

Group
BMD
Sham (Control)
0.2276 ± 0.011 a
OVX (Osteoporosis)
0.1965 ± 0.012 b
OVX + SAC
0.2276 ± 0.012 a
  • Control: sham operation
  • OVX: no treatment after ovariectomy
  • OVX+SAC: SAC treatment after ovariectomy.

The effects of Sigma Anti-bonding Molecule Calcium Carbonate on bone turnover and calcium balance in ovariectomized rats are studied. The study revealed that the induced osteoporosis was completely reversed with SAC therapy. Osteocalcin, estradiol, eosinophil, CTx and BMD level were elevated with SAC, indicating that optimal bone health is indeed restored.

Values are mean ± SD for 5 rats. Means with different superscript letters are significantly different at p<0.05 by Duncan’s multiple range tests.

"I have taken MegaGen since I was diagnosed with severe osteoporosis with T-score of -3.7. After taking 17 bottles of Megagen, my bone density is back to the normal range. Incredible!!"

M. S. PARK – Age 55, Male

Human Bone Density Clinical Case under SAC

(CBHI Canada Conducted BMD Increase Trial for +1000 Patients under SAC Therapy.)

CBHI (Calcium & Bone Health Institute of Canada) utilized FDA approved ultrasound bone densitometer by BeamMed in measuring and comparing BMD data of more than a thousand patients.  Over 90% of the patients experienced increased bone density.

Fracture Healing Effects of SAC Therapy

Steroid Induced Osteoporosis, auto fracture (Male, 52, Indonesia)

Dosage: MaraGen 2x /day for first 2 months and then only 1x. Able to walk normally again.

Decalcification Effects of SAC Therapy

Removing Calcification from Cellular to Systemic Level is a Key to Recovery

Conditions Commonly Treated with SAC Therapy

Cellular Recovery Helps to Restore Mitochondrial Function & Reduce Oxidative Stress

  • Autoimmune disease (Lupus, Vitiligo, Hashimoto’s, Crohn’s, Celiac disease, eczema, MS, rheumatoid, etc.)
  • Lyme disease, HIV, Shingles and other viral infections
  • Parkinson’s, ALS, Alzheimer’s and
    other neurodegenerative diseases
  • Arthritis, Gout, CPPD, Inflammations
  • Mitochondrial Disease
  • Cancer (carcinoma, sarcoma,
    lymphoma, leukemia, multiple
    myeloma)
  • Arrhythmia, Heart palpitation, Mitral Valve Prolapse,
  • Diabetes, Metabolic Syndrome
  • Thrombosis, Hemolytic Anemia
  • Autism Spectrum Disorder,
  • ADHD, Epilepsy
  • Asthma, COPD
  • Glaucoma, Cataract, Intermittent Exotropia, Retinal Vein Occlusion
  • Menier’s Disease, Aurora Migraine
    Disease, Tinnitus, Vertigo
  • Osteoporosis, Bone Necrosis
  • Chromosome 8 syndrome
  • Chronic Kidney Disease
  • Gum disease, Loose teeth
  • Calcification (joints and tissues),
    Calcific tendonitis, Fibrosis, Kidney
    and Gall Bladder Stones
  • Dysmenorrhea, infertility

Sigma Anti-bonding Calcium (SAC)

By | Resources, SAC

Sigma Anti-bonding Calcium (SAC)

Revolutionary Technology Brings Breakthrough Bioavailability of Ionic Calcium

SAC in a
Nutshell

SAC (Sigma Antibonding Calcium Carbonate) is the only true ionic calcium delivery system that provides calcium in free ionic state, which is the only physiologically active form of calcium in our body. Normally, calcium from diet and supplements enters our body in the protein-bound form and therefore, cannot trigger the same physiological responses as SAC. Resolving calcium deficiency better than protein-bound calcium, SAC triggers ionic-calcium-sensitive physiological responses that counteract the root cause of diseases and brings natural healing reactions of our body from cellular to the systemic level.

The Invention of New Calcium Carbonate

Sigma Anti-bonding Calcium Carbonate (SAC-CaCO3)

SAC is the world’s first calcium-ion-delivery-system, which safely and effectively elevates the level of calcium-ion concentration in our blood. By utilizing a very weak chemical bonding, namely sigma antibonding, to calcium carbonate molecules, Calcium & Bone Health Institute of Canada (CBHI) invented new calcium carbonate, which maintains loosely held calcium ion to its carbonate group. The antibonding makes the molecules exhibit electrical charge and attract water molecules via hydrogen bonding. Making sigma antibonding stable at room temperature was, in itself, a technological breakthrough after ten years of R&D.

Because of the weak chemical bonding of SAC, calcium ion is easily detached and passively absorbed into our system through stomach lining as ions via diffusion and osmotic pressure, not requiring digestion, vitamin D, nor peptides for absorption.  This is called passive transport.

Because of our body’s natural sensitivity to fluctuations of serum plasma ionic calcium level, a minimal elevation of ionic calcium concentration achieved by SAC can trigger hormonal responses, such as the release of TSH and calcitonin to trigger bone-building osteoblasts. SAC therapy utilizes ionic calcium as a signaling agent to trigger our body’s self-healing responses to reverse calcification from cellular to systemic level, causing domino effects of healing processes to rebuild our health. SAC’s healing pathway is genuinely unique.

"SAC is the world's first safe calcium-ion delivery system administered directly into our body in oral form. SAC triggers healing mechanisms no other calcium supplementation can achieve"

What is Sigma Anti-bonding?

Anti-bonding orbitals are essentially the “opposite” of bonding orbitals. They are formed when atomic orbitals combine in ways that lead to predominantly destructive interference.

 

The key feature of anti-bonding orbitals is that the molecular orbitals have a higher energy than the corresponding atomic orbitals. Thus, the molecule has a higher energy than the separated atoms (atoms separated by a large distance) and the atoms would prefer to be in the lower atomic state.

Anytime two atomic orbitals combine to give a lower-energy bonding orbital, an analogous higher energy anti-bonding orbital is also formed. Above is a figure depicting this simple bond/anti-bond molecular orbital diagram that we had for hydrogen. The diagram also shows that electrons (in this case) completely fill the bonding orbital and leave the anti-bonding orbital empty.

 

SAC calcium’s sigma anti-bonding keeps the calcium atoms in the molecule very loose and unstable. Our innovative technology locks calcium atoms in place until it is released in our body.

SAC Calcium is an innovative new bio-material based on calcium carbonate derived from small oyster shells. Highly advanced process formulates it to maintain positive charges (2+) by altering the bonding structure, namely sigma anti-bonding. The positive charges of the molecule attract water molecules to cluster around it, making it incredibly water soluble and allowing direct and passive absorption into the body.

SAC Calcium is directly absorbed because of its anti-bonding positive charge, and is immediately bio-available. SAC bypasses active transport delivery that requires digestion with peptides and vitamin D, a complicated process that leaves absorbed calcium far less bio-available.

Unique Physical Properties of SAC Calcium

Because of electrical charge of SAC calcium cabonate molecule that interacts with hydrogen bonding of water molecules, an open bottle of SAC evaporates calcium together with water molecules and crystalize with CO2 in the air.

SAC is 200x more soluable in water compared to calcium carbonate and 3x more reactive in chemical reaction.

Unique Passive Absorption of SAC Calcium

Active Transport of Regular Calcium

Regular calcium intake from diet or supplements need strong stomach acid with peptides and vitamin D3 to digest and be absorbed in small intestines as protein-bound calcium, which is not readily utilized with aging because of sedentary lifestyle, hormonal changes, and poor diet.  Various side effects of inactive calcium include kidney stones, blood vessel calcification, stroke, heart attack, etc.  Most, if not all, calcium supplements fall in this category and may aggrevate body-wide calcification which is known to be one of many major cause of degenerative diseases.

Passive Transport of SAC Calcium

Unlike regular calcium intake, no Vitamin D3 and peptides are needed for absorption. SAC diffuses passively through digestive tract cell linings (mucosa) as ions, not requiring physiological energy from our body and readily available for immediate use.

Ionic calcium is immediately utilized to bring calcium homeostasis :

  • Corrects calcium signaling
  • Reduces cellular oxidative stress
  • Restores mitochondrial function
  • Triggers decalcification

SAC is Absorbed through Diffusion & Osmotic Pressure

About 5 to 7.6 mg of SAC solution is mixed with 500ml of water for optimun absorption as ionic calcium.  SAC is takin in an empty stomach and is completely absorbed within 30 minutes through diffusion and osmotic pressure. Although it is in a small intake amount, slight elevation of ionic calcium in the blood is enough to trigger TSH (thyroid stimulating hormone) to initiate strong bone-building osteoblasts.  SAC’s cascading effects on physiological functions of our body also activate inactive protein-bound calcium in our blood to further boost and maintain good bone health.

SAC is the first calcium-ion-delivery-system to help build and support strong bones while promoting normal bone mass. SAC also plays an important role in other functions such as nerve transmission and muscular function. Unlike other electrically neutral calcium supplements that require Active Transport, SAC calcium’s Passive Transport brings full absorption of calcium without the need for Vitamin D, peptide, or other agents.

Inside our cells, SAC calcium quickly breaks down to yield calcium ions, which are absorbed into capillaries and triggers our body’s natural bone formation process.

Physiological Effects of SAC

After intake, SAC’s effect lasts about four hours in our body, initially raising the serum ionic calcium concentration to a higher yet safe level to trigger various physiological functions before bringing down the serum ionic calcium concentration down to the average physiological level.

While ionic calcium level is elevated, bone-building osteoblast with osteoclastic activity is triggered to raise the bone turnover rate, repairing and rebuilding bones. This process also activates idle protein-bound calcium, releasing both ionic calcium and protein, further fueling bone-building and clearing body-wide calcification. Ionic calcium also aids cellular metabolism, releasing more ATP (adenosine triphosphate) and raising body temperature. As kidneys try to excrete excess ionic calcium through urination, an urge to urinate within an hour of taking SAC is experienced, which is both healthy and normal, indicating that SAC is working.

STRONGER BONES LEAD TO A HEALTHIER LIFE

A long term, follow up study done in Denmark for 35,000 people revealed that the people with strong bones in their 50’s lived 11.6 years longer.
YET, in Canada, 49% of infants are born with calcium deficiency. Only 70% recover after breastfeeding. Calcium deficiency during pregnancy and infancy leads to serious health issues.

Bone Loss Leads to 150+ Degenerative Diseases

Bone health is directly related to our overall health. Emptier bone characterized by osteoporosis or osteopenia indicates not only a higher risk of fracture but also a greater chance of developing degenerative diseases. Why? Because emptying bones cause calcification in both cellular and systemic levels, causing cellular communications mayhem by disrupting calcium signaling.

Promising Animal Clinical Trials

Calcium ions in the blood are so vital that the body cannot permit it to fluctuate. Therefore, even a slight increase in the concentration of ionized calcium in the blood triggers the bone building process to take excess calcium into bones. Utilizing this process is by far the most effective and safe way to support strong bones since it follows the body’s natural bone building mechanism. This amazing effect of SAC was observed in this animal clinical trial through the bone break test where SAC ‘treated’ bone displayed almost 100x bone building power compared to regular calcium carbonate.

Importance of SAC therapy goes beyond the stronger bone-building.  Although most of our body’s calcium is stored in bone, the tiny amount that circulates in your bloodstream is disproportionately vital to good health. About half of this circulating serum calcium (50%) is “ionized”, which means it carries electrical charges and this calcium ions (Ca2+) are the only physiologically active form that can be recognized by our body and responsible for numerous functions of our body such as the firing of muscle and nerve cells, promoting blood clotting, preventing the depletion of bone mass, securing proper cellular functions by preserving calcium signaling, etc.  As we age, this vital ionic calcium homeostasis is disrupted as our bone breaks down and calcifies trillions of cells.  SAC therapy can restore this fragile calcium homeostasis and gives our body a chance to fight back the onset of 150+ degenerative diseases  that are thought to be caused by calcium displacement.

Osteoporosis Reversed under SAC Therapy

(Lab Anim Res 2011: 27(4), 301-307, 2011)

Group
BMD
Sham (Control)
0.2276 ± 0.011 a
OVX (Osteoporosis)
0.1965 ± 0.012 b
OVX + SAC
0.2276 ± 0.012 a
  • Control: sham operation
  • OVX: no treatment after ovariectomy
  • OVX+SAC: SAC treatment after ovariectomy.

The effects of Sigma Anti-bonding Molecule Calcium Carbonate on bone turnover and calcium balance in ovariectomized rats are studied. The study revealed that the induced osteoporosis was completely reversed with SAC therapy. Osteocalcin, estradiol, eosinophil, CTx and BMD level were elevated with SAC, indicating that optimal bone health is indeed restored.

Values are mean ± SD for 5 rats. Means with different superscript letters are significantly different at p<0.05 by Duncan’s multiple range tests.

Lactic Acid Reduction Clinical Trial

(Important Indicator for Effectiveness as a Cancer Treatment)

Twenty swimmers from Korea University swim team taking two doses a day for 14 days proved that SAC neutralizes lactic acid effectively. They all broke their swimming records as a result.

Lactic acid, which is the byproduct of carcinoma cancer cells, is known to inhibit the immune functions around cancer tumors. Neutralizing the lactic acid and bringing pH back to a normal level is crucial in cancer treatment.

"I have taken MegaGen since I was diagnosed with severe osteoporosis with T-score of -3.7. After taking 17 bottles of Megagen, my bone density is back to the normal range. Incredible!!"

M. S. PARK – Age 55, Male

Human Bone Density Clinical Case under SAC

(CBHI Canada Conducted BMD Increase Trial for +1000 Patients under SAC Therapy.)

CBHI (Calcium & Bone Health Institute of Canada) utilized FDA approved ultrasound bone densitometer by BeamMed in measuring and comparing BMD data of more than a thousand patients.  Over 90% of the patients experienced increased bone density.

Fracture Healing Effects of SAC Therapy

Steroid Induced Osteoporosis, auto fracture (Male, 52, Indonesia)

Dosage: MaraGen 2x /day for first 2 months and then only 1x. Able to walk normally again.

Decalcification Effects of SAC Therapy

Removing Calcification from Cellular to Systemic Level is a Key to Recovery

Conditions Commonly Treated with SAC Therapy

Cellular Recovery Helps to Restore Mitochondrial Function & Reduce Oxidative Stress

  • Autoimmune disease (Lupus, Vitiligo, Hashimoto’s, Crohn’s, Celiac disease, eczema, MS, rheumatoid, etc.)
  • Lyme disease, HIV, Shingles and other viral infections
  • Parkinson’s, ALS, Alzheimer’s and
    other neurodegenerative diseases
  • Arthritis, Gout, CPPD, Inflammations
  • Mitochondrial Disease
  • Cancer (carcinoma, sarcoma,
    lymphoma, leukemia, multiple
    myeloma)
  • Arrhythmia, Heart palpitation, Mitral Valve Prolapse,
  • Diabetes, Metabolic Syndrome
  • Thrombosis, Hemolytic Anemia
  • Autism Spectrum Disorder,
  • ADHD, Epilepsy
  • Asthma, COPD
  • Glaucoma, Cataract, Intermittent Exotropia, Retinal Vein Occlusion
  • Menier’s Disease, Aurora Migraine
    Disease, Tinnitus, Vertigo
  • Osteoporosis, Bone Necrosis
  • Chromosome 8 syndrome
  • Chronic Kidney Disease
  • Gum disease, Loose teeth
  • Calcification (joints and tissues),
    Calcific tendonitis, Fibrosis, Kidney
    and Gall Bladder Stones
  • Dysmenorrhea, infertility